Purified Anti-G4S linker Antibody[DA010] (AN011300P)

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	100μg	$ 88.00	- +
	25μg	$ 38.00	- +

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Conjugation: Unconjugated

Clone	DA010
Verified Samples	Verified Samples in FCM: HEK293T cells transfected with anti-CD19(FMC63 scFv with G4S linker) CAR
Dilution	FCM ≤ 0.2 µg per million cells in 100 µL volume
Background	The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.
Immunogen	Synthetic peptide
Abbre	G4S linker
Host	Rabbit
Clonality	Monoclonal
Purification Method	>98%, Protein A/G purified
Isotype	Rabbit IgG
Applications	FCM
Concentration	≥ 1 mg/mL
Conjugation	Unconjugated
Storage Buffer	Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Storage	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Expiration Date	12 months
Shipping	Ice bag